F810

Instrument Introduction

Detection Technology
Nucleic Acid Fluorescence Staining & Flow Cytometry to count WBC. NRBC and 6-part impedence method and flow cytometry for RBC/PLT

Detection mode
CBC, DIFF, NRBC, RET, SR

Sample mode
Whole Blood Mode, Low Value Leukocyte Mode, Predilution Mode, and Sample Research Mode

Sample volume
Whole blood mode: 88ul
Predilution mode: 70ul

Throughput
CBC+DIFF: 100T/H
CBC+DIFF+RET: 83T/H

Reporting parameters (36 in total)
Leukocyte:

WBC, NEUT(#,%), LYMPH(#,%), MONO(#,%), EO(#,%), BASO(#,%), IG(#,%)
Erythrocyte:

RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV, NRBC(#,%)
Platelets:

PLT, PDW, MPV, P-LCR, P-LCC, PCT
Reticulocytes:

RET(#,%), IRF, LFR, MFR, HER, RET-He

Auto loader
Up to 50 sample positions

Linear range
WBC: 0-500x10^9/L
RBC: 0-8.60x10^12/L
HGB: 0-260g/L
PLT: 0-5000x10^9/L

Precision

Parameter Detection Range Precision/%

WBC ≥ 3.50 × 10⁹ /L ≤2.5

RBC ≥ 3.50 × 10¹²/L ≤2.5

HGB 110 g/L – 180 g/L ≤1.0

PLT ≥ 100 × 10⁹ /L ≤4.0

HCT or MCV 30% – 50% (HCT) or 80 fL – 100 fL (MCV) ≤ 1.5 (HCT)

Principal

Special fluorescent staining solution will dye DNA or RNA blandly while 2nd Generation chemistry staining reagents will dye Enzymes/particles in cytoplasm. We know that different cells have different concentrations of DNA or RNA, which causes the depth of dying to be different. The more DNA or RNA, the stronger fluorescence signal. Since nucleic acid is the most specific part of the cell, the 3rd Generation is more sensitive to distinguish different leukocytes, especially the abnormal cells.

Combine 3rd Generation technology with flow cytometry. A single-cell stream quickly passes through a channel in the middle, and every passing cell is detected by three beams of light from three directions to get size, granularity, and nucleic acid information.

FSL (Forward Scattered Light) mainly reflects the size of the cells.
SSC (Side Scattered Light) mainly reflects the size and number of particles in cells.
SFL (Side Fluorescence Light) mainly reflects the concentration of nucleic acid.

Multiple Channels

In FCW channel, WBC, Baso, and NRBCs results will be provided. Baso and NRBCs are generated without extra reagent or cost

In FCD channel, F810 analyzer not only gives WBC 6-part differential results (with immature granulocyte),but also brings 29
research parameters

In FCR channel, 6 reticulocyte results and PLT counting (PLT-O) will be provided. PLT-O can improve the accuracy of low platelet
counting

The flow cytometry technology was used on RBC/PLT chamber which not only make more accurate RBC/PLT results but also ensure very low clog rate

LW Mode (Low White Blood Cell)

Resampling, changing channels, increasing the count by 3 times

The increase of counting particles not only makes the detection of low value have better precision, but also enables the classification of white blood cells in low value samples and the sensitive capture of juvenile cells in them, so as to avoid unnecessary risks

SR mode

Besides blood specimen, F880 also has body fluid test function without requiring dedicated reagent. The various types of body fluids include Peritoneal fluid, Pleural fluid, Cerebrospinal fluid(CSF) and Synovial fluid