top of page

Acid Naphthyl Acetate Esterase(ANAE)Stain


Intended Use

This kit is for staining bone marrow cell and bloodcell smear.


Hydrochloric acid parafuchsin reacts with Sodium Nitrite to form diazonium salt (six-azo-pararosaniline). α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form red brown precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called "Non-specific Esterase Stain".


1. Fixative(solution A)

2. Pararosaniline (solution B)

3. Sodium Nitrite (solution C)

4. PhosphateBuffer (solution D)

5. α-Naphthyl Acetate (solution E)

6. Methyl Green (solution F)

Order Information

bottom of page