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High Iron Diamine-Alcian Blue Stain



O-sulfate esters are stained brown-black with HID generated “in-situ” by the oxidation of a mixture of hydrochlorides of N, N-dimethyl meta- and para-phenylene diamines with ferric chloride. In subsequent steps, sialic acid and carboxyl groups are stained aquamarine blue with Alcian Blue at pH 2.5.


1. Alcian blue solution(pH2.5)

2. HID-solution A

3. HID-solution B

4. Ferric Chloride solution

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