Masson's Trichrome Stain
Masson's Trichrome Stain selectively stains collagen fibers. Differentiation staining of collagen fibers and muscle fibers is an important diagnostic tool in pathological diagnosis.
This method is modified from Mallory’s Trichome stain, which uses the mixture of two or three anion dyes or its function in sequence to stain the specimen. Staining results can be affected by the molecular weight of anion dyes and tissue permeability.
Demonstration of different tissue components can be made from selection of anion dyes with different molecular weight and tissue permeability.
1. Weigert's Iron Hematoxylin Solution A
2. Weigert's Iron Hematoxylin Solution B
3. Ponceau-Acid Fucshin Solution
4. Phosphomolybdic Acid Solution
5. Aniline Blue Solution