PAS Hepatin Stain
PAS Hepatin stain is intended for hepatin staining. Tissue is oxidized in periodic acid and color will develop after adding colorless Fucshin. This reaction is mainly used for examining polyose. For fungi and bacteria, the reaction also shows positive.
Periodic acid is an oxidant which can destroy carbon bond of polyrose. First, tissues are oxidized in periodic acid solution to break open the carbon bond of glycol group or ammonia hydroxyl in tissue polyose, which results in the formation of aldehyde compound. Subsequently, the free aldehyde exposed will react with colorless Fucshin to form a new red- fuchsia compound.
1. Periodic acid
2. Schiff‘s Reagent
3. Mayer Hematoxylin