Clinical evaluation sperm survival is through detecting the integrity of the sperm membrane. This kit can be routinely detected the survival rate of all sperm specimen. By dye resist staining method to identify cell membrane integrity of sperm, so as to arrive at the percentage of live sperm. Dye resisting staining technology principle is relevant based on the damage to the cell membrane, For example, a membrane in a non-live (dead) cells, allowing non penetrate the membrane dye can enter the membrane staining.