Cryptococcus Neoformans Stain
This stain is recommended for use in the identification of Cryptococcus neoformans, which possesses a gelatinous capsule.
Cryptococcus neoformans is an unicellular, encapsulated, non-motile, non-spore-forming, yeast-like fungus that possesses a thick polysaccharide cell wall and causes the infection cryptococcosis. The organism is coccus-shaped and roughly 6~8 μm in diameter. Cryptococcus Neoformans Stain is a negative staining method, which contrast a translucent, darker colored, background with stained cells but an unstained capsule. Cryptococcus neoformans, because of its large polysaccharide capsule, can be visualized by this stain. Organisms that possess a gelatinous capsule exhibit a halo around the cell against the black background created by the India ink.