Fungal fluorescence stain

Intended Use

The fungal fluorescence stain is a complex solution containing luciferin and a coloration reagent that inhibits background fluorescence. If the sample to be tested contains fungi, the fluorescence staining solution has a strong affinity with the chitin and cellulose of the cell wall of the fungus. Under the ultraviolet light (wavelength: 340 nm to 380 nm), a bright blue fluorescence is emitted. Thus morphology was observed by a fluorescence microscope, whereby qualitative detection of fungi.

Principle

The KOH contained in the reagent can be used to dissolve keratin, which helps to observe the fungal structure more clearly. As a novel stain for rapid detection of fungi, it can be used in dermatology, otorhinolaryngology, ophthalmology, respiratory medicine, ICU, etc. Examination of various suspected fungal infections, such as tinea corporis, tinea pedis, hyperthyroidism, tinea versicolor, and pulmonary fungal infections.

Applicable Sample

Dander, nail clippings, sputum, hair, pleural effusion, cerebrospinal fluid, vaginal secretions and other specimens or tissue sections.

1、Simple and fast

The operation is simple and only requires the operator to have basic knowledge of the microscope operation; Only need very short time to complete the test.

2、High sensitivity

By combining luciferin with polysaccharides such as chitin in the fungal cell wall, the reaction is sensitive. Compared with traditional direct microscopy, the positive rate is high, thus reducing the fungal detection miss detection rate.

3、High recognition

Under the microscope with clear fluorescence, the image is clear, high recognition, easy to observe, reduce the error caused by the subjective judgment of the operator.

4、Wide range of applications

Traditional methods can only detect certain fungi, but not all fungi. The fluorescent dye can combine with various fungi to emit fluorescence, including Candida, Histoplasma, and Aspergillus.