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Rapid Gram Stain

Rapid Gram Stain

Intended Use

A rapid staining procedure to classify bacteria based on their cell wall characteristic. According to the stain results, bacteria can be divided into two different groups - Gram positive (purple) and Gram negative (red).


Gram stain was devised empirically by Christian Gram, a Danish physician, in 1884. When bacteria are stained with certain basic dyes (such as the crystal violet in Rapid Gram Stain), those cells with high peptidoglycan contents (Gram-positive) in their cell wall will resist decolorization with organic solvents such as ethanol or acetone, while the Gram-negative species (with a thin layer of peptidoglycan in the cell wall) can be easily decolorized.


​1. Crystal violet

2. Iodine

3. Decolorizer

4. Fuchsin  

Order Information

Rapid Gram Stain
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