TB Stain (For pathologic Acid-Fast Stain)
This kit is based on the Ziehl-Neelsen method recommended by the WHO and it is a chemistry stain intended for the detection of acid-fast bacteria such as Mycobacterium tuberculosis.
Acid-fast bacteria such as Mycobacterium tuberculosis and Mycobacterium leprae are difficult to stain because of their lipoid capsule in the cell wall. However, once the lipoid capsule is stained with an enhanced dye such as carbolfuchsin, the newly formed compound will resist decolorization from acid-alcohol and retain the original color stained (red), which can be easily differentiated from other microorganisms that still adsorb the counter blue stain. Phenol is mordant and is able to improve the staining ability.
2. Acid Alcohol
3. Methylene Blue